regen. med. gathers momentem....

What Is A Stem Cell?
Stem cells are the body’s repair cells. They have the ability to divide and differentiate into many different types of cells based on where they are needed throughout the body. Stem cells can divide and differentiate into tissues such as skin, fat, muscle, bone, cartilage, and nerve to name a few.
Where Are They Found?
There are two basic types of stem cells; embryonic and somatic (adult). Embryonic stem cells are found in the embryo. These cells are called totipotent or pluripotent, which means they have the ability to reproduce into any mature cell type. While embryonic stem cells offer the greatest potential in differentiating into any mature cell type, there are obviously moral and ethical concerns with harvesting these cells. They also have a propensity to develop into teratomas, a type of tumor, which is a major safety concern. The second type of stem cell is the adult stem cell. These stem cells are
multipotent, which means they can still differentiate into
multiple different tissues, but unlike embryonic stem
cells, they do not develop into teratomas. Due to this,
adult stem cells have a major safety advantage over
embryonic stem cells. Adult stem cells are found
in organs and tissues, such as bone marrow,
adipose tissue (fat), skin, liver, blood vessels, and
neurons. There are no moral or ethical concerns with
harvesting adult stem cells from a patient, activating them,
and reintroducing them back into the patient.
Why Do We Use Adipose Tissue?
Adult stem cells are highly concentrated in adipose tissue (fat). There are up to 1,000 times more stem cells in a gram of fat than in a gram of bone marrow. At this concentration and with MediVet’s patented technology, it is no longer necessary to culture the stem cells to acquire the necessary cell numbers to be efficacious. Fat is also readily available in large quantities and easily accessible. For example, the procedure to extract fat from a dog is much quicker and less invasive than a spay. The stem cells are extracted from the fat among a mixture of cells termed the Stromal Vascular Fraction (SVF). The SVF is rich in complementary cells and bioactive peptides that contribute to cell proliferation and tissue regeneration, and also impart anti-inflammatory effects. These are benefits lost once stem cells are cultured.Stem cells from adipose tissue have been shown to differentiate into many different cell types. For all the above reasons, fat is an ideal source of stem cells. Results of clinical trials currently support the use of adipose-derived stem cells for treatment of orthopedic conditions such as joint injuries, ligament and tendon damage, fractures, and osteoarthritis. Research is ongoing in a very wide range of diseases and disorders, with promising early results in:
- Inflammatory Bowel Disease - Pancreatitis
- Degenerative Myelopathy - Urinary Incontin
- Atopy
Capabilities Of Adipose-
Derived Stem Cells
Capabilities of Adipose Stem CellsMuscleBoneCartilageAdiposeNeuronAdipose-Derived Mesenchymal Stem CellsMyocardiumEndotheliumPancreasLiver
Stem Cell Theorized Mode Of Action
Adipose-derived stem cells, also called Mesenchymal stem cells (MSCs) are known to exert anti-proliferative, immunomodulatory, and anti-inflammatory effects. MSCs exert profound immunosuppression by inhibiting T-cell proliferation in response to various stimuli in vitro. They induce regulatory immunosuppressive lymphocytes and CD8 apoptosis. MSCs inhibit cell cycle progression and CD4 allo-proliferation. Several factors contained in MSCs may mediate these effects: TGF-ß, hepatocyte growth factor, prostaglandin E2, and interleukin-1 receptor antagonist. MSCs enhance proliferation, migration and differentiation of endogenous stem cells in most tissues of the body. This mode of action likely functions through excretion of chemokines and cytokines; and enhancement of growth through cell-to-cell contact and mitochondrial exchange may also have a role.
What Is Platelet-Rich Plasma?
Platelet-Rich Plasma, or PRP, is blood plasma that contains concentrated amounts of platelets and growth factors. PRP is a non-surgical and advanced means of providing high doses of the body’s own healing factors right in the area they are needed.
Growth Factors In PRP:
PDGF (Platelet derived growth factor)
TGF-aß (Transforming growth factor alpha & beta)
EGF (Epidermal growth factor)
FGF (Fibroblast growth factor)
IGF (Insulin growth factor)
PDEGF (Platelet derived epidermal growth factor)
PDAF (Platelet derived angiogenesis factor)
IL-8 (Interleukin-8)
TNF-a (Tumor necrosis factor alpha)
CTGR (Connective tissue growth factor)
GM-CSF (Granulocyte macrophage colony stimulating factor)
KGF (Keratinocyte growth factor)
Histamines, Serotonin, ADP, Thromboxane A2, and other vasoactive and
chemotactic agents
High platelet concentration and native fibrinogen concentration for
improved hemostasis
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Why Do We Use PRP?
Not only does PRP contain growth factors that have been shown clinically to aid in soft and hard tissue healing, but PRP has also been shown to enhance the proliferation of adipose-derived stem cells. Therefore, when combined with stem cells, PRP provides the nutrients necessary to “supercharge” the healing process.
Activated Stem Cells and Stromal Vascular Fraction:
Adipose tissue extracted from the patient is broken down by enzymes then filtered and centrifuged to obtain cells termed the Stromal Vascular Fraction (SVF). The SVF contains many beneficial proteins, including:
MediVet uses its patented Light-Emitting Diode (LED) technology to activate the adipose-derived stem cells before reintroducing them back into the patient. LED-activation has been shown to increase the proliferative capacity of the cells through the utilization of photobiostimulation. This process is prior to injection.
ADSC Cell Viability (ATP Luminescence)
T
ime: 0hr 24hr 48hr
Control 628+28 942+83 1109+27
LED 594+121 1233+227* 1507+281*
PRP 722+93 1221+56* 1562+38*
PRP+LED 767+74 1646+101** 2232+82**
Cells were incubated in patients PRP and or activated with laser for 30 min. Dulbecco’s Modified Eagle Medium (DMEM), 10% foetal bovine serum (FBS), 0.1% penicillin/ streptomycin, 1 µg/ml Fungizone. The cultures were incubated at 37°C in an atmosphere of 5% carbon dioxide (CO).
• High concentration of leukocytes (neutrophils, eosinophils) for microbicidal events
• High concentration of wound macrophages and other phagocytic cells, for biological debridement
• Thymosin beta-4 (controls T cell maturation/helps damaged cells repair themselves)
• Osteoblast-stimulating factor 1 (bone formation)
• Caveolin-1 (liver regeneration and lipogenesis)
• Ephrin-A1 (involved in the developing nervous system and erythropoietin)
• Connective tissue growth factor (wound healing, skin fibroblast stimulator/endothelial angiogenesis)
• Transforming growth factor beta-2 (major growth factor)
• The SVF also contains the various proteins present in the adipose tissue extracellular matrix. Laminin is of interest due to its ability to help in neural regeneration.
LED + PRP = Maximized Results
By utilizing MediVet’s patented LED technology, along with Platelet-Rich Plasma from the patient, we are able to provide the most potent cell preparation on the market today.
Activation techniques increase success & staying power.
Administering The Stem Cells
After the patient’s stem cells have been mixed with the PRP and activated, they are re-administered back to the patient. The cells are injected intra-articularly (IA) into the affected joints, and/or given intravenously (IV) for systemic healing. A portion of the cells can also be banked for future use. By maximizing the stem cell count, you have more options for treating now and in the future. Most patients will not need another injection on average for 2-3 years following treatment. By banking the stem cells now, your patient can avoid surgery for future stem cell therapy.
Where Do The Stem Cells Go After They Are Administered?
We inject some of the stem cells locally, when feasible such as in a joint, to concentrate the stem cells and PRP at the site of injury. We also administer some of the cells systemically. When infused intravenously, ADMSCs travel to damaged tissue throughout the body by following chemotactic distress signals produced by injured tissue (eg stromal-cell-derived factor 1 (SDF-1) and fractalkine). Research has shown that stem cells will cross both a healthy and an unhealthy blood-brain barrier. While clinical studies are still ongoing, we have already seen clinical improvement with other co-morbidities such as allergies, IBD, urinary incontinence, kidney and liver damage to name a few. The next few years will be very exciting as more research is conducted to find other diseases that can be treated with stem cell therapy.
Where The Stem Cells Go:
Duplex quantitative.......................Stem Cell Therapy Results
We expect you to see results! While we know every animal is different and there are no guarantees, we have seen positive clinical improvement in 95% of the arthritic cases treated nationwide. Some owners have even reported seeing a difference in as little as a week! While quick results are possible, we expect you to begin seeing improvement within the first 90 days following treatment. Chronic osteoarthritis may require multiple injections, so banking your extra cells is always a good idea!
What Other Vets Are Saying:
“The results of our first three patients have sold us on the MediVet-America process. Response of our patients and satisfaction of their human families has been outstanding.
Adopting on-site collection, processing and
administration has even improved employee
morale. It has helped us to successfully care
for patients with improved outcome,
increased efficiency and lower cost for our
clients. It has truly been win-win-win and
we look forward to helping many more
dogs in the Chicagoland area.”
- M. Robbins, DVM, DACVS
Veterinary Specialty Center Buffalo Grove, IL
“Offering Stem Cell therapy might be the best
thing I have done since I became a Vet... no, it’s
probably the best thing I will do in my career!”
- Dr. Anita McMillen, V.M.D.
Braden Run Animal Hospital
Dr. Mike Hutchinson
Dr. Joseph Yocum
Dr. Brian T. Voynick
University of Melbourne
University of Sydney
Colorado State University
The Garvin Institute
University of New South Wales
All Related References, Case Studies,
and Additional Research Documents
can be found at
www.MediVet-America.com/science.html
Addtional Research Documents
• A. Boquest et.al. ‘Isolation and transcription profiling of purified uncultured human stromal cells...
• A.Banas et.al ‘IFATS Collection In Vivo Therapeutic Potential of Human Adipose...
• A.I.Caplan ‘Mesenchymal stromal cells tissue repair and immune modulation’
• A.Nasef et.al ‘Immunosuppressive effects of mesenchymal stem cells- involvement of HLA-G’
• Activation of Adipose
• B.Mvula et.al. ‘effect of low level laser irradiation etc...
• B.Mvula et.al. ‘effect of low level laser irradiation...
• D.D.Frisbie et.al. ‘Treatment of experimental equine osteoarthritis by in vivo delivery...
• Effect of laser therapy on attachment, proliferati...[Biomaterials. 2005] - PubMed Result
• Effect of low-level laser irradiation and epiderma...[Lasers Med Sci. 2009] - PubMed Result
• Fact_Sheet_5_-_Stem_Cell_Research_in_Australia
• Fact_Sheet_7_-_Induced_Pluripotent_Stem_Cells
• G.E.Kilroy ‘Cytokine profile of human adipose-derived stem cells Expression of angiogenic, hematopoietic, and pro-inflammatory factors’
• Goat Case Study 1
• Goat Case Study 2
• J.Gimble et.al. ‘Adipose-derived stem cells for regenerative medicine’
• J.M. Murphy et.al. ‘Stem cell therapy in caprine model of osteoarthritis’
• K. LeBlanc ‘Mesenchymal stromal cells tissue repair and immune modulation’
• K.Yoshimura et.al. ‘Characterization of freshly isolated and cultured cells derived from...
• L.A.Loeb et.al ‘The mitochondrial theory of aging
• L.Black et.al ‘Effect of Adipose-Derived Mesenchymal Stem and Regenerative Cells on Lameness in Dogs with Chronic Osteoarthritis of the Coxofemoral Joints’
• low level laser ADSC EGF
• M.Varma et.al ‘Phenotypical and functional characteristics of freshly isolated...
• molecular-laser-stems 1423-0127-16-4
• P.A.Zuk et.al. Multilineage cells from human adipose tissue implications for cell...
• T.E.Meyerrose et.al.’ In vivo transplantation of human adipose-derived...
• The effect of low level laser irradiation on adult...[Lasers Med Sci. 2008] - PubMed Result
• The effect of low level laser irradiation on impla...[Swed Dent J Suppl. 2005] - PubMed Result
• Types of stem cells
Thanks To Our Partners In Stem Cell Research Cheers Vin